In plant cells, the concentration of ions and other materials is significantly higher in the vacuole than in the cytoplasm. This accumulation against the concentration gradient is facilitated by which of the following?
Options:
- A: Passive diffusion through the tonoplast
- B: Simple osmosis across the cell wall
- C: Facilitated diffusion via aquaporins
- D: Active transport facilitated by proteins in the tonoplast
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